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Bone morphogenetic protein can be induced to enhance the osteogenic potential of bone marrow and allogeneic bone

Follow-up period of 3 months to 6 years, with an average of 44 months follow-up of more than 3 years 28 patients (36 hips), 18 patients (22 hips) F ic cases at stage I, II, hip no significant pain and dysfunction, more than 3 years follow-up of 28 patients (36 hips) the hip Ha rris score, the mean preoperative (4 ± 9) minutes, mean postoperative (7 ± 7. 2) points (P <0. 0 1). The authors believe that the method of using bone morphogenetic protein can induce to enhance the osteogenic potential of bone marrow and allogeneic bone allograft fibula to provide direct mechanical support for block development and collapse of the femoral head lesions, bone allograft bone morphogenetic protein and autologous bone marrow can be used as the treatment of femoral F ic at Ⅰ, Ⅱ of avascular necrosis of a choice.

 

Bone marrow stromal cells is a commonly used seed cells for bone tissue engineering

In the early stage of fracture, soft tissue defects, systemic disease causes new blood vessels will lead to delayed union or nonunion. Bone after transplantation, the activity of vascular granulation tissue ingrowth of bone grafts, bone graft is absorbed, take the initiative to the formation of new bone. However, in the large segment of bone grafts, this process slow and incomplete. The key to these problems lies in the revascularization of bone problems that plagued mankind for a long time.

VEGF is a multifunctional cytokine secreted by platelets, megakaryocytes, endothelial cells and osteoblasts, and its role throughout human reproduction, development, tissue regeneration, repair, and tumor pathophysiological process. Many recent studies have shown that also play an important role of VEGF in bone formation and repair, the expression level directly affect the future into the bone's blood supply, affect the speed and quality of the osteoblast.

VEGF was the introduction of bone tissue engineering technology is bound to promote new bone formation, increase the speed and quality of new bone. Exogenous VEGF in vivo half-life is short, and expensive, its application bring great inconvenience. Bone marrow stromal cells is a commonly used seed cells for bone tissue engineering, it contains many cells such as endothelial cells into fiber cells, macrophages, etc. can be secreted expression of VEGF in. How to stimulate the bone marrow stromal cells to promote the secretion of VEGF is another important means of expression of VEGF

 

Bone sialoprotein osteogenesis mechanisms and bone sialoprotein osteogenic effect by Wang et al in 1998 first reported the osteogenesis of bone sialoprotein - collagen complex in the rat skull defect model, bone sialoproteinacid protein in the role of osteoblasts in bone defect model gradually attracted attention. O 'Toole, bone sialoprotein coating of metal implants, the rat femur implantation experiments, the results also proved that bone sialoprotein has good bone induction. Wang Jinxi, also confirmed the osteogenic activity of bone sialoprotein in rats. The results suggest that bone sialoprotein in bone defect showed osteogenic activity and induction of mature bone tissue, but at the same dose of the osteogenic activity of bone morphogenetic protein, possibly because of the bone sialoprotein acid protein in the tissue calcification in the performance of dual regulation, on the one hand, to promote hydroxyapatite aggregate to form nuclei, on the other hand it can be adsorbed on the surface of hydroxyapatite crystals showed inhibition of hydroxyapatite crystal growth characteristics, may be because of the contradictory role of bone sialoprotein in tissue calcification osteogenic effect was weaker than larger doses of bone morphogenetic protein.

 

bone sialoprotein as a novel bone growth factor requires further study

In this study, bone sialoprotein by the People's Liberation Army, Guangzhou Military Region, Guangzhou General Hospital of Section, Wang Jie, the human bone sialoprotein gene was cloned into Pichia pastoris expression vector pPICZ αA, the expression of a fusion protein of recombinant human bone salivaacid protein, the recombinant human bone sialoprotein protein of high purity, single component, the cost is greatly reduced, reducing the purification of bone sialoprotein may be mixed with other bone growth factors to produce the error. β-tricalcium phosphate is a classic alternative filling materials, with good osseointegration, degradation and bone conduction, bone defects and absorbed as new bone replaced. Β-tricalcium phosphate used in this experiment after eight weeks has been almost completely absorbed, 60% of its porosity, pore size 100 to 500 μm can be used as a good scaffolds for tissue engineering bone.

The present study demonstrated that bone sialoprotein in osteoblast activity and bone morphogenetic protein contrast, the osteogenic mechanism is different, the same dose, the effectiveness of bone sialoprotein osteoblast bone morphogenetic protein weak bone defects in the organization engineered bone as the Orthopaedic Research hotspots, bone sialoprotein as a novel bone growth factor requires further study, due to the osteogenic mechanism is not fully understood and require further experiments to confirm.

 

Recombinant human bmp-2 combined with basic fibroblast growth factor promotes vascular egf expression and osteogenic potential of bone marrow stromal cells in rabbits

        In the early stage of fracture, because of soft tissue defects, disease causes new blood vessels will lead to delayed union or nonunion. Bone after transplantation, the activity of vascular granulation tissue ingrowth of bone grafts, bone graft is absorbed, take the initiative to the formation of new bone. However, in the large segment of bone grafts, this process slow and incomplete. The key to these problems lies in the revascularization of bone problems that plagued mankind for a long time. Angiogenesis is an important part of the fracture healing process, a good blood supply to the fracture site of great significance in fracture repair. Vascular endothelial growth factor (vascularendothelial growth factor, VEGF) is a multifunctional cytokine, one of the most directly promotion of angiogenesis research has shown that VEGF play an important role in bone formation and bone metabolism, mainly through the proliferation of vascular generated in bone development form.Many recent studies have shown that VEGF is mainly derived from vascular endothelial cells is an important mediator of angiogenesis induced by multiple cytokines. On the other hand, increased expression levels of these cytokines will lead to increased expression of VEGF or synthetic. Various cytokines to promote bone efficacy and VEGF expression in varied ways, better application of what kind of cytokines, or combined with the feasibility of a variety of cytokines, there is no clear conclusion.

       Bone tissue engineering, bone tissue implanted ability to grow well in the host of the important issues and problems, but also the main purpose of this experiment is located. Bone tissue engineering if reasonable the joint use of hBMP-2 and bFGF and other cell factors will promote bone marrow stromal cells in the rapid proliferation and osteoblast conversion, promoting vascular endothelial cells have wire split and enhanced their differentiation, and thus the promotion of engineering bone have an important role in the host blood vessels formation and survival of the engineered bone.

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